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M94A2061.TXT
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1994-10-24
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Document 2061
DOCN M94A2061
TI The role of adhesion molecules in HIV infection.
DT 9412
AU Gomez PM; Hildreth EK; Johns Hopkins University, School of Medicine,
Department of; Pharmacology and Mol Sciences, Baltimore, Maryland 21205.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):39 (abstract no. 129A). Unique
Identifier : AIDSLINE ICA10/94370514
AB OBJECTIVE: The goal of this research is to determine the role that
adhesion molecules play in HIV-1 infection of primary cells. METHODS:
HIV-1 isolates RF and MN were grown in Jurkat cells and used to infect
PHA stimulated peripheral blood mononuclear cells. Plasma samples
obtained from two normal vaccinated individuals hyperimmunized with
recombinant HIV/MN gp120 from Genetech were tested in neutralization
assays to determine their ability to neutralize the HIV/MN lab strain.
Antibodies to CD4 and LFA-1 were used in combination with vaccinated
sera to determine synergistic effect of inhibition. HIV production was
determined by assaying for the viral core antigen (p24) by ELISA. Data
is represented as percent of inhibition in comparison to virus
production when no antibodies are present. RESULTS: Vaccinated sera V17
and V424 were diluted 1:4 and 1:40 and cultured with HIV/MN and PHA
blasts in the presence and absence of LFA-1 antibody (H52 20 ug/ml). As
displayed in the graph there was a synergistic inhibition of viral
replication when HIV was cultured in the presence of vaccinated sera and
LFA-1 antibody. In addition, the effect of antibody to LFA-1 was the
same regardless of the dilution of vaccinated sera. CONCLUSION: We
conclude that adhesion molecules can play a role in HIV infection. HIV
has been reported to acquire molecules which are expressed on the host
cell surface during budding. Therefore, the quantity and the type of
molecules acquired by the virus would be determined by the type of cell
it buds. We speculate that primary isolates would acquire a greater
degree of adhesion molecules because they would be budding from
activated cells. We are currently performing neutralization assays to
determine if vaccinated sera and adhesion molecules would neutralize
primary isolates to a greater extent than lab-isolates. TABULAR DATA,
SEE ABSTRACT VOLUME.
DE AIDS Vaccines/IMMUNOLOGY Cell Adhesion Molecules/*IMMUNOLOGY/ISOLATION
& PURIF Enzyme-Linked Immunosorbent Assay Human HIV Core Protein
p24/IMMUNOLOGY HIV-1/*IMMUNOLOGY Leukocytes, Mononuclear/MICROBIOLOGY
Lymphocyte Function-Associated Antigen-1/IMMUNOLOGY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).